浮游植物metacaspase的分布、结构及其功能特性     

苏金净  蔡伟聪  李桂玲  李健  曾珺  刘静雯 《海洋与湖沼》2019,50(5):1059-1069

精氨酸/赖氨酸特异性半胱氨酸蛋白酶(Metacaspase)在浮游植物程序性细胞死亡(programmed cell death.PCD)过程中起着关键作用。本文利用生物信息学方法分析metacaspase基因在浮游植物中的分布,并以海洋球石藻(Emilianiahuxleyi)metacaspases(EhMCs)为代表分析其结构和功能,依据Uniprot和String数据库挖掘与metacaspase功能相关并参与浮游植物PCD过程的互作蛋白。结果发现:共计29条、四种类型的metacaspases分布于11种浮游植物中,它们的metacaspase蛋白一级结构存在明显差异,但位于p20的组氨酸和半胱氨酸活性位点均具有高度的保守性;EhMCs三级结构与酵母的metacaspase十分相似,暗示它们具有类似的功能;浮游植物metacaspases与多种蛋白存在直接或间接的互作关系,在细胞PCD过程、响应环境胁迫和能量代谢等方面发挥重要作用。另外.metacaspase的磷酸化修饰可能在浮游植物PCD信号转导过程中起调节作用。  相似文献   

Experimental study on hydrodynamic performance of SWATH vessels in calm water and in head waves     

《Applied Ocean Research》2019

An extensive experimental investigation on four SWATH hull forms has been conducted in calm water and in regular waves at University of Naples Federico II. Calm water tests have been analyzed in the range of Froude number Fr_T from 0.1 to 0.6. For all four SWATH configurations at the speed, corresponding to Fr_T 0.32, the behaviour in regular waves has been tested. The results of heave, pitch and vertical accelerations are presented in nondimensional form as RAO. For the “most promising” SWATH #4 configuration, a set of stabilizing fins have been designed and an active stabilization system has been developed. The developed SWATH#5 has been tested in calm water on three displacements in the range of Fr_T from 0.1 to 0.65. The dynamic wetted surface has been identified and the residual resistance coefficient C_R as well as R_T/Δ are reported. Seakeeping tests have been performed in regular head sea and in head and following irregular sea at Fr_T = 0.50. The conditions for the occurrence of dynamic longitudinal instabilities have been identified. The results allows to comment the effect of slenderness of struts and SWATH’s immersed bodies on resistance and seakeeping and concerns the applicability of SWATH concept to small craft.  相似文献   

Uncertainty in marine weather routing     

《Applied Ocean Research》2019

Weather routing methods are essential for planning routes for commercial shipping and recreational craft. This paper provides a methodology for quantifying the significance of numerical error and performance model uncertainty on the predictions returned from a weather routing algorithm. The numerical error of the routing algorithm is estimated by solving the optimum path over different discretizations of the environment. The uncertainty associated with the performance model is linearly varied in order to quantify its significance. The methodology is applied to a sailing craft routing problem: the prediction of the voyaging time for an ethnographic voyaging canoe across long distance voyages in Polynesia. We find that the average numerical error is an order of magnitude smaller than the performance model uncertainty. These results illustrate the significance of considering the influence of numerical error and performance uncertainty when performing a weather routing study.  相似文献   

Research on the Methods of Performance Monitoring and Evaluation for Active Hydrogen Maserstwo     

《Chinese Astronomy and Astrophysics》2019,43(2):292-304

Active hydrogen maser is the main frequency standard for establishing and maintaining the time scale. It has the characteristics of high short-term stability and low phase noise. At present, it plays an increasingly important role in the international atomic time (TAI) and various local time scales. Firstly, in combination with the internal state parameters of an active hydrogen maser, the correlation between the internal state parameters and the comparison data of a hydrogen maser is analyzed, and a method for the performance monitoring of a hydrogen maser is proposed. Secondly, according to the characteristics of hydrogen maser performance, a method for evaluating the performance of a hydrogen maser is given. The hydrogen maser performance includes mainly two aspects, namely the frequency stability and predictability. The performances of two types of active hydrogen masers (CH1-75 and MHM-2010) are evaluated by this method. The correlation analysis of the atomic clock state parameters and comparison data shows that the state parameter monitoring can effectively predict the variation of the hydrogen maser performance. The evaluation results of atomic clock frequency stability and predictability show that the atomic clock with a higher medium-and-long term frequency stability has a better predictability. There are two methods for the predictability evaluation, one is based on the data published by BIPM (Bureau International des Poids et Measures), and another one is based on the quadratic model. Both methods are consistent with the weights published by BIPM. Therefore, the two methods can be used as a quantitative method to evaluate the predictability of a hydrogen maser.  相似文献   

基于手机数据的北京市城市与近郊交互模式挖掘     

彭卉  杜云艳  易嘉伟  刘张  王会蒙 《地球信息科学学报》2019,21(1):97-106

在城镇化进程中,城市与近郊之间通过职住、货运、游憩等活动产生越来越紧密的交互联系,对于这些交互联系的准确识别和定量刻画,是理解城乡空间关系的重要手段,也能为城市的资源有效配置与合理规划提供重要的现状信息。本文通过对全北京在一日之内的手机信令数据所反映的人群移动轨迹的深入分析,融合城市的POI信息形成顾及人类活动时空信息的空间交互类型推断。以北京市为例,对城市中心与近郊之间远距离的强交互进行定性、定量和定位的探索。本文发现了北京市多尺度下空间交互模式和距离衰减规律,判断了城乡异常交互类型,对比了城乡之间和城市内部的交互模式的异同,以及基于交互类型视角提取了城乡异常交互的空间特征。研究认为,基于手机信令数据,利用停留点提取和高斯核密度估计的空间交互类型推断有效地发现了北京市周末的远距离出行类型特点,提取了其空间交互强度和空间特征,揭示了基于人类活动的北京市周末城乡交互模式。  相似文献   

Isolation, distribution and evaluation of cytotoxic and antioxidant activity of cultivable actinobacteria from the Oman Sea sediments     

Gozari Mohsen  Bahador Nim  Jassbi Amir Rez  Mortazavi Mohammad Seddiq  Hamzehei Sam  Eftekhar Ebrahim 《海洋学报(英文版)》2019,38(12):84-90

Screening bioactive natural products from bacteria is a determinative step in the drug discovery programs. The present study aim to isolate actinobacteria from the Oman Sea sediments for determining the effects of different culture media and treatments on the yield of the isolation process, and measure the DPPH radical scavenging and Artemia cytotoxic activity of culture extracts of the actinobacterial isolates. A total of 290 actinobacterial isolates were collected from 14 sediment samples. Heat treatment(40.68%) and M_4 medium(29.31%) exhibited the maximum isolation rates of actinobacteria. Streptomyces isolates were dominantly distributed in all of the investigated stations according to 16 S rRNA gene sequencing. The distribution pattern of Streptomyces followed a depth-dependent frequency trend, whereas the members of rare genera including Micromonospora, Nocardia Actinoplanes, Nocardiopsis, Saccharopolyspora and Crossiella were distributed in deeper stations. Approximately,25% of the examined isolates could scavenge 90% of 10~(–4) mol/L DPPH solutions at 1 250 μg/mL final concentration of their ethyl acetate culture extracts. Furthermore, the most potent extracts could scavenge DPPH radicals with IC50 ranges from 356.8 to 566.4 μg/mL. Brine shrimp cytotoxicity tests showed that 38.88% of the examined culture extracts exhibited LC_(50) lower than 1 000 μg/mL against the Artemia cells. Moreover, the most potent culture extracts exhibited LC_(50) range from 335.4 to 534.4 μg/mL. Phylogenetic analysis by 16 S rRNA gene sequence revealed that the OS 005, OS 263 and OS 157 closely related to Streptomyces djakartensis, Streptomyces olivaceus and Nocardiopsis dassonvillei respectively. These results suggested the widespread distribution of the antioxidant and cytotoxic producing actinobacteria in the Oman Sea sediments, which could be considered as promising candidates for the discovery of microbial bioactive compounds.  相似文献   

时间尺度重构EEMD-GRNN改进模型预测PM2.5的研究     

符海月  张祎婷 《地球信息科学学报》2019,21(7):1132-1142

合理构建PM_2.5浓度预测模型是科学、准确地预测PM_2.5浓度变化的关键。传统PM_2.5预测EEMD-GRNN模型具有较好的预测精度,但是存在过于关注研究数据本身而忽略其物理意义的不足。本研究基于南京市2014-2017年PM_2.5浓度时间序列数据,分析PM_2.5浓度多尺度变化特征及其对气象因子和大气污染因子的尺度响应,基于时间尺度重构进行EEMD-GRNN模型的改进与实证研究。南京市样本数据PM_2.5浓度变化表现为明显的天际尺度和月际尺度,从重构尺度（天际、月际）构建GRNN模型更具有现实意义;同时,PM_2.5对PM₁₀、NO₂、O₃、RH、MinT等因子存在多尺度响应效应,以其作为GRNN模型中的输入变量更具有时间序列上的解释意义。改进后的EEMD-GRNN模型具有更高的PM_2.5浓度预测精度,MAE、MAPE、RMSE和R²分别为6.17、18.41%、8.32和0.95,而传统EEMD-GRNN模型的模型有效性检验结果分别为8.37、27.56%、11.56、0.91。对于高浓度天（PM_2.5浓度大于100 μg/m³）的预测,改进模型更是全面优于传统EEMD-GRNN模型,MAPE为12.02%,相较于传统模型提高了9.03%。  相似文献   

GRB 170817A的洛伦兹因子和观测角          下载免费PDF全文

林一清 《天文学报》2020,61(1):6

伴随着引力波事件GW170817的短暴GRB (Gamma-Ray Burst) 170817A首次提供了双中子星并合与短暴相联系的直接证据.但是短暴GRB 170817A具有非常弱的光度,意味着观测的视线方向可能偏离喷流轴方向.根据短暴静止系的峰值能量E_(p,i)和各向同性光度L_(iso)。之间的关系以及洛伦兹因子Γ和L_(iso)。之间的关系估算了短暴GRB 170817A以及长短暴GRB 060614观测角与喷流边缘的夹角θ'_(obs)和洛伦兹因子Γ,结果表明GRB 170817A的Γ=45±27,θ'_(obs)=2.2±0.5°,而GRB 060614的Γ=214±93,θ'_(obs)=0.5±0.1°.这个结果相当于GRB 170817A的正轴各向同性光度L_(iso,on)=(2.1±0.7)×10~(49) erg·s~(-1),比典型的短暴少2-3个数量级.GRB 060614的L_(iso,on)=(5.12±1.91)×10~(51) erg·s~(-1)与典型短暴相当.这意味着GRB 060614可能属于短暴类型,而GRB 170817A可能本质上就是一个弱暴.  相似文献   

蛭龙活血通瘀胶囊及其拆方对AS大鼠ET-1、NO的影响          下载免费PDF全文

郑利珠  张诗意  廖慧玲 《应用海洋学学报》2020,36(12):139

目的：观察蛭龙活血通瘀胶囊及其拆方对动脉粥样硬化（AS）大鼠内皮素（ET）-1和一氧化氮（NO）的影响,并探讨其组方的合理性。方法：随机将110只健康雄性SD大鼠分为11组,分别为空白组、模型组、阿托伐他汀组、全方组和拆方1~7组,每组各10只,除空白组外其余各组均制备AS大鼠模型。阿托伐他汀组大鼠予以阿托伐他汀钙片干预,全方组予以黄芪、桂枝、大血藤、地龙、水蛭干预,拆方1组予以黄芪、桂枝、大血藤干预,拆方2组予以黄芪、地龙、水蛭干预,拆方3组予以黄芪干预,拆方4组予以桂枝、地龙干预,拆方5组予以桂枝、水蛭干预,拆方6组予以大血藤、地龙干预,拆方7组予以大血藤、水蛭干预,于实验第120天后检测各组大鼠治疗前后的血清ET- 1、NO水平。结果：蛭龙活血通瘀胶囊全方组较拆方组降低血清ET-1浓度和提高血清NO浓度的作用更为明显,诸药中影响大鼠血清ET-1、NO含量最显著的是黄芪。结论：蛭龙活血通瘀胶囊全方组中多种药味合用后,对降低AS大鼠血清ET-1和提高NO含量有明显的协同作用,方中多种药物均可保护内皮功能,且影响大鼠血清ET-1、NO含量最显著的药味是黄芪。  相似文献   

桂林-阳朔地区DEM地形特征与岩性相关性分析及分类研究     

陈霄燕  潘军  邢立新  蒋立军  孙也涵  仲伟敬  范博文 《地球信息科学学报》2019,21(12):1867-1876

地形地貌是岩性解译的重要信息,地形因子作为描述DEM数字曲面几何特征的定量指标参数,可用来定量化表达不同岩性所在地区地形地貌特征。本文以桂林-阳朔地区为研究区,研究地形因子数学、地质意义,建立岩性与地形因子组合间的定量关联,进而实现岩石类型划分。本文基于ASTERGDEM提取坡度、起伏度等12个地形因子,在分析各个地形因子地质意义基础上,通过聚类分析及方差分析的多元统计分析方法,研究各岩性地形因子特性及其关联性,建立研究区岩性之间的定量差异;此外,利用因子分析方法研究岩性分类过程中的主导因素,确定适宜岩性分类方法以实现定量化岩性分类。实验结果表明：不同岩性、不同地形地貌的地形因子（组合）之间具有显著差异,基于因子分析得到的宏观地形复杂度指数（MTI）以及微观曲率指数（MCI）对岩石类型的分类精度达77.36%。研究表明,地形复杂度等地形因子可用于岩性分类,采用因子分析方法可获取反映地形地貌宏观、微观特征的定量指标,且岩性分类效果良好。  相似文献